INSERM U963 / CNRS UPR9022Navigation
Tools for Anopheles gambiae Transgenesis. Volohonsky G.*, Terenzi O.*, Soichot J.*, Naujoks D.A., Nolan T., Windbichler N., Kapps D., Smidler A.L., Vittu A., Costa G., Steinert S., Levashina E.A., Blandin S.A. and Marois E., 2015 – Genes, Genomes, Genetics 13;5(6):1151-63 pubmed Abstract Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest...Read More
A brand new, larger and more functional insectarium will be constructed starting in 2015. The project is funded by the government-impulsed “Plan Campus“, of which the University of Strasbourg is a laureate. The new building will triple the space currently dedicated to mosquito breeding and highly-secured laboratories, in which experiments involving the human malaria parasite Plasmodium falciparum and mosquito-borne viral pathogens will be possible. By extending our current research on the mouse malaria model to the human malaria parasite, and to the transmission of viral pathogens by mosquitoes, the new insectary will allow us and our colleagues working on Drosophila to answer questions directly relevant to human...Read More
INSERM U963 / CNRS UPR9022 - Strasbourg, France
About 20 species of mosquitoes in the genus Anopheles are known vectors of Plasmodium, transmitting the parasite while taking a blood meal on humans. Among them, Anopheles gambiae is the major malaria vector in sub-Saharan Africa. Importantly, mosquitoes are not mere needles that transmit the parasites, they actively fight the parasite. In some mosquitoes, parasite development is even completely blocked early after infection, making these mosquitoes unable to transmit the disease. We aim at understanding the genetic basis of the resistance/susceptibility of mosquitoes towards Plasmodium, and we develop new tools for efficient mosquito transgenesis to investigate mosquito-parasite interactions.Left photos : GFP-expressing parasites are visible on the midgut of a mosquito from the susceptible strain while mosquitoes from the resistant strain are devoid of live parasites. Right photo : transgenic larvae of a strain we call “french kiss”.